WebThe complete protocol of blue-white screening includes 3 important steps: Ligation: ligation of foreign DNA into MCS of the plasmid vector. Transformation: introduction of plasmid … WebCloning plasmid vector overview. Cleave vector with restriction enzyme. Gene of interest is cut out of foreign DNA with same restriction enzymes. Mix vector and DNA fragment, …

Traditional Cloning Basics Thermo Fisher Scientific - US

WebThis video explains how blue-white screening is an important tool in molecular biology to detect the recombinant clones WebMethod #2: A powerful way. An effective method to simplify screening is to use a positive selection system – a twist on the blue/white system mentioned above. Positive selection vectors conditionally express a lethal gene, such as a restriction enzyme that digests the genomic DNA of the bacterial host. When your DNA is successfully inserted ... other ways to charge laptop

Blue-White Screening & Protocols for Colony Selection

WebFigure 2. Cloning of human IGF in pUC57-Kan cloning plasmid vector and blue-white colony screening. (A) The gene insert, human IGF, was ligated into the MCS of pUC57 … WebCloning Vector pUC18 Product Information Sheet # V33002 MoBiTec GmbH, Germany Phone: +49 551 70722 0 Fax: +49 551 70722 22 E-Mail: [email protected] www.mobitec.com Revised January 2014 1 SUMMARY Product pUC18 high copy cloning vector for replication in E.coli, suitable for “blue-white screening” technique. Description The blue–white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a suitable bacterial strain, but other organisms such as yeast may also be used. DNA of transformation is ligated into a vector. The vector is then inserted into a compet… other ways of saying replied