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WebInvitrogen™ Bolt™ 4-12% Bis-Tris Plus gels were loaded with HeLa lysate ranging from 10 to 50 µg and electrophoresed using MES running buffer. Proteins from the gels were transferred onto mini PVDF membranes using the Invitrogen ™ iBlot 2 Gel Transfer Device with iBlot 2 Transfer Stacks (P0 protocol for 7 minutes). WebBolt™ Bis-Tris Plus 8%, 10%, 12%, 4–12% 6 to 400 kDa up to 16 months 22–45 min Best choice for separation of small- to medium-sized proteins. Neutral pH environment … how to get the index column of a dataframe
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