2024 Ddr1 assay

Ddr1 assay

Author: mtab

August undefined, 2024

WebKinase Activity Assays Count on minimal interference from your compound library with luminescent, ADP-based kinase assays. These sensitive and reliable assays are easily scaled to meet your throughput needs. Select from over 370 Kinase Enzyme Systems, including >100 mutant kinases. Learn about Kinase Activity Assays Shop Kinase … WebFeb 9, 2024 · To examine whether the interaction depends on DDR1 kinase activity, we introduced DDR1-K618A for Co-IP assay. The results suggested that the interaction …

PathHunter® eXpress DDR1 Functional Assay

WebLanthaScreen® Eu Kinase Binding Assay for DDR1 Overview This protocol describes how to perform a LanthaScreen® Eu Kinase Bindng Assay designed to detect and … WebDiscoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. ikea bathurst

DDR1-IN-1 ≥99%(HPLC) Selleck DDR inhibitor QCFile

WebPurpose: Discoidin domain receptor 1 (DDR1) belongs to a novel class of receptor tyrosine kinases. Previous evidence indicates that DDR1 overexpression promotes the … WebJan 12, 2024 · DDR1 has five isoforms namely: DDR1a, DDR1b, DDR1c, DDR1d, and DDR1e. Amongst these; DDR1a, DDR1b, and DDR1c are kinase-active containing the KD, and they are full-length functional receptors, whereas DDR1d and DDR1e are kinase-inactive, due to truncation or frame shifts, so they are nonfunctional receptors [ 19 ]. WebAug 20, 2024 · The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for the extracellular matrix protein collagen. The interactions of cells with … is there diamonds in every chunk

The GPER-1 regulates cAMP/PKA/p-CREB pathway. (A) The

Kinase Activity Assays - Promega

WebThis study reports the identification of a potent DDR1 inhibitor, nilotinib, for the treatment of breast cancer. MTT assay was used to evaluate the inhibitory activity of nilotinib and meantime we used flow cytometry to evaluate the pro-apoptotic activity of nilotinib in MCF-7 and MDA-MB-231 cells. WebDiscoidin domain receptor family, member 1, also known as DDR1 or CD167a (cluster of differentiation 167a), is a human gene. Function. Receptor tyrosine kinases (RTKs) play … is there dick cancerWebJun 28, 2024 · b: Colony formation assay after knockdown of DDR1 in OEC-M1 and TW2.6 cells for 10 days (left). The mean number of colonies for each well was determined from three independent assays (right). c: Growth rates of OEC-M1 and TW2.6 cells measured by MTT assay after DDR1 knockdown. ikea bath sink cabinet

"WebHuman DDR1 ELISA Kit (ab260063) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DDR1 protein in cell culture supernatant. It uses our … " - Ddr1 assay

Ddr1 assay

DDR1 promotes breast tumor growth by suppressing …

WebTo further investigate the effects of DDR1 on tumor cell growth in vivo, a tumorigenesis assay was performed in BALB/c female mice using 4T1 cells with or without stable DDR1 overexpression. The tumors in the … WebSep 2, 2024 · Next, we investigated the DDR1 inhibitory activity of compound 1 and compound 2 as measured by autophosphorylation in U2OS cells. The compounds showed IC 50 values of 10.3 and 5.8 nM,...

Did you know?

WebDDR1-IN-2 (DDR1 inhibitor 7rh) is a potent, selective, ATP-competitive Discoidin domain receptor 1 (DDR1) inhibitor (IC50: 6.8 nM in cell-free kinase assays). DDR1-IN-2 inhibited the enzymatic activity of DDR1, with IC50 values of 6.8 nM, but were significantly less potent in suppressing the kinase activities of DDR2, Bcr-Abl, and c-Kit. It ... WebPhospho-DDR1 (Tyr792) Antibody recognizes endogenous levels of DDR1 protein only when phosphorylated at Tyr792. This antibody may cross-reacts with other tyrosine …

WebAssay Properties Assay Format 384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay ... DDR1 NanoBRET Kinase Assay. Reference Compound IC50 for DDRI 120 100 40 -10 Dasatini -6 -5 Log[Dasatinib] (M) -0.8910 6.991e-009 HILLSLOPE EC50 . Title: View PDF

WebGene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay. PrimePCR™ SYBR® Green Assay: DDR1, … WebNational Center for Biotechnology Information

WebPathHunter® DDR1 Functional Assay, DiscoverX DiscoverX offers over a decade of experience in generating stable cell lines of unsurpassed quality and leading performance utilizing the proprietary enzyme fragment complementation (EFC) technology against a broad range of target classes (GPCRs, kinases, epigenetics targets (methyltransferases ...

WebSep 21, 2024 · The Discoidin Domain Receptor 1 (DDR1) is one of the two members of a unique family of receptor tyrosine kinase receptors that signal in response to collagen, … ikea bathtub trayWebProduct name DDR1 Matched ELISA Antibody Pair Set,Human Assay type Solid Phase Sandwich ELISA Specificity Quantitative determination of Human DDR1 Assay range 56.25-3600 pg/mL Materials provided 1. Capture Antibody 0.5 mg/mL of rabbit anti-Human DDR1 / MCK10 / CD167 monoclonal antibody (in PBS, pH 7.4). is there diesel near meWebDDR1 Kinase Assay Service Activity-based DDR1 kinase assay for compound screening and profiling via radiometric HotSpot TM kinase assay. Target Overview CAT# DDR1 … is there diapers for dogsWebPathHunter® DDR1 Functional Assay, DiscoverX; ... to deliver optimal assay performance. The Starter Pack catalog number has the number 4 in front of the cell line catalog number (e.g. for the cell line Cat. No. 93-0787C2, the appropriate Starter Pack Cat. No. is 493-0787C2). Please refer to the cell line-specific datasheet for the appropriate ... is there diamonds in the netherWebAdditionally, the results of the gain- and loss-of-function of the DDR1 assay in MC3T3-E1 cells indicated that DDR1 can regulate the osteoblast differentiation through activating autophagy by ... is there diet grape crushWebAssay Protocol HEK293 cells transiently expressing NanoLuc-DDR1 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-4 and then treated with reference compound Dasatinib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. is there dietary fiber in meatWebReport: A detailed report including assay conditions and comprehensive evaluation of data as well as raw data for each analysis will be provided. Screening location: Malvern, PA, USA Compound requirements: In brief, 50 to 100 µl of a 10 to 50 mM DMSO stock or 2-3 mg solid material is needed. is there dictation on google docs