WebNov 21, 2008 · Fcp1 is a Y-shaped protein composed of an acylphosphatase domain located at the base of a deep canyon formed by flanking modules that are missing from the small CTD phosphatase (SCP) clade: an Fcp1-specific helical domain and a C-terminal BRCA1 C-terminal (BRCT) domain. WebFcp1 purification DNAs encoding S. pombe Fcp1(140-580), Fcp1(140-580)-D170N, or Fcp1(149-580)-∆330-393 were inserted into pSMT3 (Mossessova and Lima, 2000), so as to fuse the Fcp1 gene in frame with a leader sequence encoding an N-terminal His 6-Smt3 tag. Missense mutations were introduced into the pSMT3-FCP1(140-580) plasmids by PCR
Sub1 and RNAPII, until termination does them part
WebMar 3, 2005 · Fcp1 in yeast is chromatin associated both at the promoter and the coding region and has been suggested to function in ser 2 dephosphorylation ( Cho et al, 2001 ). Fcp1 thus appears to play an important role during transcription elongation. WebJan 30, 2009 · The diagnostic PCR results, using primers targeting sequences outside the gene deletion construct, confirm the formation of heterokaryons containing both parental and deleted alleles. The deletion strains of the other 23 phosphatase genes were confirmed to be viable homokaryons, demonstrating the function of these protein phosphatases to … the jesus freak is super weak song
The Structure of Fcp1, an Essential RNA Polymerase II
WebFormation of this complex is strongly driven by hydrophobic interactions in the minimal binding motif. Here, we test the hypothesis that charged residues in Fcp1 that flank the … WebMar 21, 2024 · FCP1 (F-Cell Production 1) is a Genetic Locus. Diseases associated with FCP1 include Fetal Hemoglobin Quantitative Trait Locus 3 and Sickle Cell Disease . … WebAug 1, 2005 · The CNG technique is particularly suitable for capturing dynamic protein-protein interactions as exemplified here by the formation and demonstration of RNA polymerase II-Fcp1 complex. Publication types Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. MeSH terms the jesus church of colorado springs