Webythdc1（也称剪切因子 yt521）可作为外显子增加或外显子跳读的一个关键调节分子来调节选择性剪切。ythdc1 可通过将前 mrna 剪切因子 srsf3 募集到含 m6a 的区域来促进外显子增加，同时通过阻断 srsf10 结合这些相同区域来抑制外显子跳读 (2)。 Web6 aug. 2024 · Zhang et al. (2024) show that in the absence of either YTHDC1 or METTL3, or when METTL3 activation by ATM is impaired, DNA damage accumulates. Furthermore, they find a delayed recruitment of the homology-directed recombinational repair factors RAD51 and BRCA1. As a consequence, loss of m6A causes sensitivity to DNA damaging …
Suppression of m6A mRNA modification by DNA hypermethylated
Web对于一组circRNA来说，这种增加是由于m6A机制的表达增加，同时发现m6A机制控制RMS细胞的增殖活性。此外，作者认为发现RNA解旋酶DDX5是反向剪接反应的调节因子，也是m6A调控网络的辅助因子。DDX5和m6A reader YTHDC1被证明相互作用，并促进RMS中circRNAs的公共子集的产生。 WT and Ythdc1 cKO ES cells were plated onto 6-well dishes at a density of 2 × 105 cells per well. Serum/LIF medium supplemented with 1 μM 4OHT, 4 μg ml−1 polybrene, and WT or mutant Ythdc1 viral supernatants were simultaneously added into the corresponding wells. After 24 h of infection, fresh … Vedeți mai multe CD-1 mice were used as embryo donors and the pseudopregnant recipients for blastocyst injection. Animal experiments were … Vedeți mai multe Male mouse ES cells were derived from 3.5 days post coitum dpc inner cell mass (ICM) from 129 mice (stock no. 217, Beijing Vital … Vedeți mai multe Mouse Ythdc1, Ythdc1-mut, Ythdc1-W378A, Ythdc1-W429A and Setdb1 were cloned into the pRlenti-3× Flag vector. Ythdc1 and Setdb1 were also cloned into the pRlenti-HA vector. For transient expression, … Vedeți mai multe We applied CRISPR–Cas9 system to construct the gene-editing cell lines in male 129 mouse ES cells. The specific guide RNA (gRNA) sequences targeting genome were designed and inserted into the pX330 … Vedeți mai multe reseal marble shower
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Web7 nov. 2024 · Recently, it was found that m 6 A is closely related to APA. However, the molecular mechanism of this new APA regulation remains elusive. Here, we show that YTHDC1, a nuclear m 6 A reader, can suppress proximal APA sites and produce longer 3' UTR transcripts by binding to their upstream m 6 A sites. WebHere, we discovered that the m6A reader Ythdc1 is indispensable for mouse skeletal muscle regeneration and proliferation of muscle stem cells. In the absence of Ythdc1, … WebWang et al. reveal that N6-methyladenosine (m6A) modification of the long noncoding RNA MALAT1 is critical for the metastatic ability of cancer cells both in vitro and in vivo. MALAT1-m6A recognition by YTHDC1 regulates the composition and function of nuclear speckles, thereby amplifying cancer gene expression. reseal mouthwash bottle