Primescript reverse transcription reagent kit
Webit takes a while to start reverse transcription reaction after the reaction mixture was prepared. The synthesized cDNA can be used for many experiments such as 2nd-strand ... Composition of Supplied Reagent : 5X PrimeScript II Buffer (for cDNA synthesis ) 250 mM Tris-HCl, pH 8.3 375 mM KCl 15 mM MgCl2 Standard protocol for 1st-strand cDNA ... WebUSD $1067.00. This kit for two-step RT-PCR is designed to provide excellent extendibility and efficient amplification. It uses PrimeScript reverse transcriptase and Takara Ex Taq …
Primescript reverse transcription reagent kit
Did you know?
WebPrimeScript RT reagent Kit is designed to perform the reverse transcription optimized for real-time RT-PCR. It uses PrimeScript Reverse Transcriptase, which features excellent … WebSYBR PrimeScript miRNA RT PCR Kit was used to measure the expression of miR-139-5p. The corresponding primers of miR-139-5p and U6 were acquired from Applied Biosystems. Two micrograms of total RNA underwent reverse transcription (RT) to generate cDNA with the help of PrimeScript RT Reagent Kit using oligo (dT) primers (Takara). SYBR Green …
WebThe v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT) receptor tyrosine kinase is a type 3 transmembrane receptor for mast cell growth factor (MGF) also known as stem cell factor). It was originally isolated as a retroviral oncogene (v-kit) from the acute transforming Hardy-Zuckerman 4 feline retrovirus. WebReverse Transcription Kit Primescripttm Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …
WebApr 13, 2024 · Qualitative reverse transcription PCR assays confirmed the responsiveness of OsjDIR genes to the ... The extraction of Total RNA and the synthesis of cDNA were performed using a KKFast Plant RNApure Kit (ZOMANBIO) and PrimeScript™ RT reagent kit (TaKaRa), respectively. The qRT-PCR reaction system (10 μL) contained 5μL (2 × ... WebOct 29, 2024 · Total RNA was extracted from VP-incubated cells utilizing the TRIzol regent (Invitrogen; Thermo Fisher Scientific, Inc.) using established protocol. cDNA was synthesized using the PrimeScript reverse transcription reagent kit (TIANGEN BIOTECH Co., Beijing, China) by following established guidelines. cDNA was assessed using qPCR on a …
WebReal-time PCR kits. Probe-based qPCR compounds. 5X One-Step PrimeScript III RT-qPCR; One-Step PrimeScript RT-PCR Builder (Perfect Real Time) An Step PrimeScript III RT-PCR Kit; PrimeDirect Probe RT-qPCR Mix; Premix Ex Taq (Probe qPCR) Premix Ex Taq DNA Polymerase (Perfect Real Time) CellAmp Direct Probe RT-qPCR Kit; Measuring qPCR Mix …
WebReal-time PCR kits. Probe-based qPCR mixes. 5X One-Step PrimeScript III RT-qPCR; One-Step PrimeScript RT-PCR Kit (Perfect Real Time) One Step PrimeScript III RT-PCR Kit; PrimeDirect Probe RT-qPCR Mix; Premix Ex Taq (Probe qPCR) Premix Ex Taq DNA Polymerase (Perfect Real Time) CellAmp Direct Probe RT-qPCR Kit; Dye-based qPCR … sunday school camping themeWebThe TRIzol reagent (Invitrogen) was used to extract the total RNA content from the RCC cells. The extracted RNA content was treated with a PrimeScript RT assay kit (Takara Bio, Inc., Tokyo, Japan) for isolation of the complementary DNA. Next, qPCR was performed on the ABI7500 real-time PCR system using SYBR green (Takara Bio, Inc.). sunday school central john mcclendonWebRequest bulk or custom quote. Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT which maintains activity at 42-50°C and is suitable for synthesis of cDNA up to 13 kb. sunday school books for childrenWebThe ProtoScript ® II First Strand cDNA Synthesis Kit is optimized mix for higher specificity and yield of cDNA. Recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability (active up to 48°C) Generates cDNA up to 10 kb. Supplied with Oligo-dT primers, Randomized Primer Mix, and nuclease-free water. palm coast fl internet providersWebThis reverse transcription reagent kit facilitates cDNA synthesis prior to analysis by real-time PCR. Designed for two-step real-time RT-PCR (also called qRT-PCR or RT-qPCR), the … palm coast fl massagesWebUSD $380.00. PrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. It uses PrimeScript Reverse Transcriptase, which … palm coast fl bridal shop in european villageWebMar 1, 2024 · PrimeScript ™ RT reagent Kit (Perfect Real Time) 品牌 Code No. ... Fast reverse transcription from mouse blood samples using PrimeScript Reverse … palm coast fl is in which county